HSI-II Gene Cluster of Pseudomonas syringae pv. tomato DC3000 Encodes a Functional Type VI Secretion System Required for Interbacterial Competition.
Identifieur interne : 000144 ( Main/Exploration ); précédent : 000143; suivant : 000145HSI-II Gene Cluster of Pseudomonas syringae pv. tomato DC3000 Encodes a Functional Type VI Secretion System Required for Interbacterial Competition.
Auteurs : Ching-Fang Chien [Taïwan] ; Cheng-Ying Liu [Taïwan] ; Yew-Yee Lu [Taïwan] ; You-Hsing Sung [Taïwan] ; Kuo-Yau Chen [Taïwan] ; Nai-Chun Lin [Taïwan]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2020.
Abstract
The type VI secretion system (T6SS) is a widespread bacterial nanoweapon used for delivery of toxic proteins into cell targets and contributes to virulence, anti-inflammatory processes, and interbacterial competition. In the model phytopathogenic bacterium Pseudomonas syringae pv. tomato (Pst) DC3000, two T6SS gene clusters, HSI-I and HSI-II, were identified, but their functions remain unclear. We previously reported that hcp2, located in HSI-II, is involved in competition with enterobacteria and yeast. Here, we demonstrated that interbacterial competition of Pst DC3000 against several Gram-negative plant-associated bacteria requires mainly HSI-II activity. By means of a systematic approach using in-frame deletion mutants for each gene in the HSI-II cluster, we identified genes indispensable for Hcp2 expression, Hcp2 secretion and interbacterial competition ability. Deletion of PSPTO_5413 only affected growth in interbacterial competition assays but not Hcp2 secretion, which suggests that PSPTO_5413 might be a putative effector. Moreover, PSPTO_5424, encoding a putative σ54-dependent transcriptional regulator, positively regulated the expression of all three operons in HSI-II. Our discovery that the HSI-II gene cluster gives Pst DC3000 the ability to compete with other plant-associated bacteria could help in understanding a possible mechanism of how phytopathogenic bacteria maintain their ecological niches.
DOI: 10.3389/fmicb.2020.01118
PubMed: 32582082
PubMed Central: PMC7283901
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Interbacterial Competition.</title><author><name sortKey="Chien, Ching Fang" sort="Chien, Ching Fang" uniqKey="Chien C" first="Ching-Fang" last="Chien">Ching-Fang Chien</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Agricultural Chemistry, National Taiwan University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Cheng Ying" sort="Liu, Cheng Ying" uniqKey="Liu C" first="Cheng-Ying" last="Liu">Cheng-Ying Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Agricultural Chemistry, National Taiwan University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Yew Yee" sort="Lu, Yew Yee" uniqKey="Lu Y" first="Yew-Yee" last="Lu">Yew-Yee Lu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Agricultural Chemistry, National Taiwan University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Sung, You Hsing" sort="Sung, You Hsing" uniqKey="Sung Y" first="You-Hsing" last="Sung">You-Hsing Sung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Agricultural Chemistry, National Taiwan University, 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Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The type VI secretion system (T6SS) is a widespread bacterial nanoweapon used for delivery of toxic proteins into cell targets and contributes to virulence, anti-inflammatory processes, and interbacterial competition. In the model phytopathogenic bacterium <i>Pseudomonas syringae</i> pv. tomato (<i>Pst</i>) DC3000, two T6SS gene clusters, HSI-I and HSI-II, were identified, but their functions remain unclear. We previously reported that <i>hcp2</i>, located in HSI-II, is involved in competition with enterobacteria and yeast. Here, we demonstrated that interbacterial competition of <i>Pst</i> DC3000 against several Gram-negative plant-associated bacteria requires mainly HSI-II activity. By means of a systematic approach using in-frame deletion mutants for each gene in the HSI-II cluster, we identified genes indispensable for Hcp2 expression, Hcp2 secretion and interbacterial competition ability. Deletion of PSPTO_5413 only affected growth in interbacterial competition assays but not Hcp2 secretion, which suggests that PSPTO_5413 might be a putative effector. Moreover, PSPTO_5424, encoding a putative σ<sup>54</sup>-dependent transcriptional regulator, positively regulated the expression of all three operons in HSI-II. Our discovery that the HSI-II gene cluster gives <i>Pst</i> DC3000 the ability to compete with other plant-associated bacteria could help in understanding a possible mechanism of how phytopathogenic bacteria maintain their ecological niches.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32582082</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>HSI-II Gene Cluster of <i>Pseudomonas syringae</i> pv. tomato DC3000 Encodes a Functional Type VI Secretion System Required for Interbacterial Competition.</ArticleTitle><Pagination><MedlinePgn>1118</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2020.01118</ELocationID><Abstract><AbstractText>The type VI secretion system (T6SS) is a widespread bacterial nanoweapon used for delivery of toxic proteins into cell targets and contributes to virulence, anti-inflammatory processes, and interbacterial competition. In the model phytopathogenic bacterium <i>Pseudomonas syringae</i> pv. tomato (<i>Pst</i>) DC3000, two T6SS gene clusters, HSI-I and HSI-II, were identified, but their functions remain unclear. We previously reported that <i>hcp2</i>, located in HSI-II, is involved in competition with enterobacteria and yeast. Here, we demonstrated that interbacterial competition of <i>Pst</i> DC3000 against several Gram-negative plant-associated bacteria requires mainly HSI-II activity. By means of a systematic approach using in-frame deletion mutants for each gene in the HSI-II cluster, we identified genes indispensable for Hcp2 expression, Hcp2 secretion and interbacterial competition ability. Deletion of PSPTO_5413 only affected growth in interbacterial competition assays but not Hcp2 secretion, which suggests that PSPTO_5413 might be a putative effector. Moreover, PSPTO_5424, encoding a putative σ<sup>54</sup>-dependent transcriptional regulator, positively regulated the expression of all three operons in HSI-II. Our discovery that the HSI-II gene cluster gives <i>Pst</i> DC3000 the ability to compete with other plant-associated bacteria could help in understanding a possible mechanism of how phytopathogenic bacteria maintain their ecological niches.</AbstractText><CopyrightInformation>Copyright © 2020 Chien, Liu, Lu, Sung, Chen and Lin.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chien</LastName><ForeName>Ching-Fang</ForeName><Initials>CF</Initials><AffiliationInfo><Affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Cheng-Ying</ForeName><Initials>CY</Initials><AffiliationInfo><Affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Yew-Yee</ForeName><Initials>YY</Initials><AffiliationInfo><Affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sung</LastName><ForeName>You-Hsing</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Kuo-Yau</ForeName><Initials>KY</Initials><AffiliationInfo><Affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Nai-Chun</ForeName><Initials>NC</Initials><AffiliationInfo><Affiliation>Department of Agricultural Chemistry, National Taiwan University, Taipei, 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name="Taïwan"><noRegion><name sortKey="Chien, Ching Fang" sort="Chien, Ching Fang" uniqKey="Chien C" first="Ching-Fang" last="Chien">Ching-Fang Chien</name></noRegion><name sortKey="Chen, Kuo Yau" sort="Chen, Kuo Yau" uniqKey="Chen K" first="Kuo-Yau" last="Chen">Kuo-Yau Chen</name><name sortKey="Lin, Nai Chun" sort="Lin, Nai Chun" uniqKey="Lin N" first="Nai-Chun" last="Lin">Nai-Chun Lin</name><name sortKey="Liu, Cheng Ying" sort="Liu, Cheng Ying" uniqKey="Liu C" first="Cheng-Ying" last="Liu">Cheng-Ying Liu</name><name sortKey="Lu, Yew Yee" sort="Lu, Yew Yee" uniqKey="Lu Y" first="Yew-Yee" last="Lu">Yew-Yee Lu</name><name sortKey="Sung, You Hsing" sort="Sung, You Hsing" uniqKey="Sung Y" first="You-Hsing" last="Sung">You-Hsing Sung</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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